Isolation of Pathogenic Bacteria on injury site of the Skin and the assessment of antimicrobial resistance pattern among patients living in Birnin Kebbi city, Kebbi State Nigeria

Abstract

This study was carried out with the aim of isolating bacterial pathogens from wound infection as well as assessing the antimicrobial resistance pattern, with emphasis of assessing multiple drugs resistance pathogenic bacteria. Random sampling techniques was used to generate Data of detecting pathogens and their antimicrobial resistivity of the bacterial pathogens species in infected wounds specimens including buruli ulcer, watery substances derided tissues, aspirates and swabs received from people living with wounds around Birnin kebbi city, from September 2021 to January 2022). Out of sixty two (62) samples of wound swabs that was investigated through Microscopy, Culturing,and Sensitivity for Microbiological assessment of Bacterial pathogens from wound infection. 46(75.85%) shows bacterial growth and 16(24.1%) of the samples turns out to be negative (no growth of bacterial pathogens was observed). Bacterial pathogens isolated from specimens, 17 (27.4%) indicates the growth of S. aureus, 10(16.1%) indicates E. coli growth, 8( 12.9%) indicates growth of pseudomonas spp, 3(4.8%) for Klebsiella spp, 3(4.8%) for Proteus 1(1.6%) for Stresptococcus spp , 1(1.6%) for Enterobacter spp, and 3(4.8%) for Citrobacter spp. Wound infection according to gender , out of the sixty two (62) samples, Male patients recorded the highest with 36( 58.0%) of bacterial growth followed by 26(41.9%) indicating the bacterial growth for the female patients. The bacterial isolated from wound include Staphylococcus aureus, Streptococcus, pseudomonas, Enterobacter are resistance to Amoxicillin while Proteus, Citrobacter, E. coli and Enterobacter were highly resistant to Pefloxacin and Cefotaxime. The treatment of wound infection with antibiotics without completing the dosage results to the transformation of certain bacterial to secret enzymes that will be inactivating antimicrobial substance enabling the growth certain organisms.